Mouse monoclonal [AD1.1.10] antibody to His Tag (AP).
Specificità
This antibody recognises proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Applicazioni
WB, ELISA
Diluizioni consigliate
WB: 1:500 - 1:1,000
Reattività
Species Independent
Immunogeno
PAX6 transcription factor linked to His tag.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
AD1.1.10
Isotipo
IgG1
Coniugare
AP
Purificazione
Protein A affinity chromatography of tissue culture supernatant.
Concentrazione
500 µg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Conservazione
Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.
Note generali
Mouse anti Histidine tag antibody, clone AD1.1.10 recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Sinonimi
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
