Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Adenosine 5'-monophosphoramidase HINT1, Desumoylating isopeptidase HINT1, HINT, Histidine triad nucleotide-binding protein 1, PKCI-1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1
Western blot analysis of various lysates, using Anti-HINT1 Antibody (A306843) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence analysis of H9C2 cells using Anti-HINT1 Antibody (A306843) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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