Anti-HIF-1 alpha Anticorpo [HIF1A/3248] (A315082)

$765

Anticorpo monoclonale [HIF1A/3248] di topo contro HIF-1 alpha per IHC-P.

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Spese di spedizione
Tempi di consegna
4-6 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Prodotti consigliati per Anti-HIF-1 alpha Anticorpo

Nome
Anti-HIF-1 alpha Antibody [HIF1A/3248]
Descrizione
Mouse monoclonal [HIF1A/3248] antibody to HIF-1 alpha.
Specificità
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Applicazioni
IHC-P
Controllo positivo
Human breast or bladder carcinomas.
Diluizioni consigliate
IHC: 1-2 µg/ml
Reattività
Human
Immunogeno
Recombinant fragment (aa 239-530) of human HIF-1 alpha protein.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
HIF1A/3248
Isotipo
IgG1
Catene leggere
kappa
Coniugare

Unconjugated

Purificazione
Protein A/G chromatography.
Concentrazione
200 µg/ml
Peso molecolare
92-110 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
ARNT interacting protein, ARNT-interacting protein, Basic helix loop helix PAS protein MOP1, Basic-helix-loop-helix-PAS protein MOP1, bHLHe78, Class E basic helix-loop-helix protein 78, HIF 1A, HIF 1alpha, HIF-1-alpha, HIF1, HIF1 A, HIF1 Alpha, HIF1-alpha, HIF1A, HIF1A_HUMAN, Hypoxia inducible factor 1 alpha, Hypoxia inducible factor 1 alpha isoform I.3, Hypoxia inducible factor 1 alpha subunit, Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor, Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor), Hypoxia inducible factor1alpha, Hypoxia-inducible factor 1-alpha, Member of PAS protein 1, Member of PAS superfamily 1, Member of the PAS Superfamily 1, MOP 1, MOP1, PAS domain-containing protein 8, PASD 8, PASD8
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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