HER2 (phospho Tyr1248) Cell Based Kit Elisa (A103792)

Name: HER2 (phospho Tyr1248) Cell Based ELISA Kit
Brand: Antibodies.com
SKU: A103792
Price: 645.0 USD
Availability: InStock

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$645

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Nome

HER2 (phospho Tyr1248) Cell Based ELISA Kit

Tipo di kit

Cell-based (qualitative)

Principio di kit

This HER2 (phospho Tyr1248) Cell Based ELISA Kit allows for the detection of HER2 (phospho Tyr1248) and the effects that certain stimulation conditions have on HER2 (phospho Tyr1248) expression in different cell lines. Qualitative determination of HER2 (phospho Tyr1248) concentration is achieved by an indirect ELISA format. In essence, HER2 (phospho Tyr1248) is captured by Anti-HER2 (phospho Tyr1248) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-HER2 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Piattaforma

Microplate

Tipo di rilevamento

Fluorometric

Campo di rilevamento

> 5000 Cells

Reattività

Human, Mouse, Rat

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at + 4°C. Stable for 6 months.

Sinonimi

C erb B2/neu protein, CD340, CD340 antigen, Cerb B2/neu protein, CerbB2, Erb b2 receptor tyrosine kinase 2, ErbB 2, ERBB2, ERBB2_HUMAN, HER 2, HER 2/NEU, Herstatin, Human epidermal growth factor receptor 2, Metastatic lymph node gene 19 protein, MLN 19, MLN19, NEU, NEU proto oncogene, Neuro/glioblastoma derived oncogene homolog, Neuroblastoma/glioblastoma derived oncogene homolog, NGL, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase type cell surface receptor HER2, Tyrosine kinase-type cell surface receptor HER2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog), V erb b2 avian erythroblastic leukemia viral oncoprotein 2, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573)

Western blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 30', using Anti-HER2 (phospho Tyr1248) Antibody.

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Figure 2: HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573)

A diagram showing the protocol for HER2 (phospho Tyr1248) Cell Based ELISA Kit.

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Figure 3: HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573)

A summary of the protocol for HER2 (phospho Tyr1248) Cell Based ELISA Kit.

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Figure 4: Western Blot - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573)

The mouse monoclonal antibody to GAPDH is used as a positive control in HER2 (phospho Tyr1248) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.