HER2 (phospho Tyr1112) Cell Based Kit Elisa (A102214)

Name: HER2 (phospho Tyr1112) Cell Based ELISA Kit
Brand: Antibodies.com
SKU: A102214
Price: 645.0 USD
Availability: InStock

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$645

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Nome

HER2 (phospho Tyr1112) Cell Based ELISA Kit

Tipo di kit

Cell-based (qualitative)

Principio di kit

This HER2 (phospho Tyr1112) Cell Based ELISA Kit allows for the detection of HER2 (phospho Tyr1112) and the effects that certain stimulation conditions have on HER2 (phospho Tyr1112) expression in different cell lines. Qualitative determination of HER2 (phospho Tyr1112) concentration is achieved by an indirect ELISA format. In essence, the HER2 (phospho Tyr1112) is captured by Anti-HER2 (phospho Tyr1112) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this HER2 (phospho Tyr1112) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-HER2 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Adherent cells and suspension cells.

Campo di rilevamento

> 5000 Cells

Tempo del kit

4 hours 30 minutes

Reattività

Human, Mouse, Rat

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at + 4°C. Stable for 6 months.

Sinonimi

C erb B2/neu protein, CD340, CD340 antigen, Cerb B2/neu protein, CerbB2, Erb b2 receptor tyrosine kinase 2, ErbB 2, ERBB2, ERBB2_HUMAN, HER 2, HER 2/NEU, Herstatin, Human epidermal growth factor receptor 2, Metastatic lymph node gene 19 protein, MLN 19, MLN19, NEU, NEU proto oncogene, Neuro/glioblastoma derived oncogene homolog, Neuroblastoma/glioblastoma derived oncogene homolog, NGL, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase type cell surface receptor HER2, Tyrosine kinase-type cell surface receptor HER2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog), V erb b2 avian erythroblastic leukemia viral oncoprotein 2, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - HER2 (phospho Tyr1112) Cell Based ELISA Kit (CBP1724)

Western blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 20', using Anti-HER2 (phopho Tyr1112) Antibody.

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Figure 2: HER2 (phospho Tyr1112) Cell Based ELISA Kit (CBP1724)

A diagram showing the protocol for HER2 (phospho Tyr1112) Cell Based ELISA Kit.

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Figure 3: HER2 (phospho Tyr1112) Cell Based ELISA Kit (CBP1724)

A summary of the protocol for HER2 (phospho Tyr1112) Cell Based ELISA Kit.

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Figure 4: Western Blot - HER2 (phospho Tyr1112) Cell Based ELISA Kit (CBP1724)

The mouse monoclonal antibody to GAPDH is used as a positive control inHER2 (phospho Tyr1112) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.