Anti-Hemopexin Anticorpo [ARC1444] (A305314)

Western blot analysis of extracts of Mouse lung, using Anti-Hemopexin Antibody [ARC1444] (A305314) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 2min.

Western blot analysis of extracts of Human serum, using Anti-Hemopexin Antibody [ARC1444] (A305314) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-Hemopexin Antibody [ARC1444] (A305314) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat liver using Anti-Hemopexin Antibody [ARC1444] (A305314) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).