Rabbit polyclonal antibody to Hemoglobin subunit alpha.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hemoglobin subunit alpha (Hemoglobin subunit alpha (HBA1)) (NP_000549.1).
Western Blot - Anti-Hemoglobin subunit alpha Antibody (A91546)
Western blot analysis of extracts of K-562 cells, using Anti-Hemoglobin subunit alpha Antibody (A91546) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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