Anti-Heme Oxygenase 1 Anticorpo [6B8-2F2] (A304770)

Western blot analysis of Human, mouse, rat Rat kidney Lysate showing detection of ~31 kDa HO-1 protein using Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat kidney Lysate. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was HRP Goat Anti-Mouse at 1:50 for 1 hour at room temperature. Color Development: TMB solution for 5 minutes at room temperature. Predicted/Observed Size: ~31 kDa.

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus, Cytoplasm. Magnification: 60X.