Western blot analysis of extracts of Rat liver, using Anti-HDAC6 Antibody (A80508) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-HDAC6 Antibody (A80508) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Anti-HDAC6 Antibody (A80508) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 100µg extracts of HepG2 cells using 3µg of Anti-HDAC6 Antibody (A80508). This Western blot was performed on the immunoprecipitate using Anti-HDAC6 Antibody (A80508) at a dilution of 1:1000.
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