Anti-HDAC4 (phospho Ser632) Anticorpo [ARC56258] (A305405)

Western blot analysis of extracts of Jurkat, using Anti-HDAC4 (phospho Ser632) Antibody [ARC56258] (A305405) at 1:2,000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.