The antibody detects endogenous level of HDAC4 / HDAC5 / HDAC9 only when phosphorylated at serine 246 / 259 / 220.
Applicazioni
WB, IHC
Diluizioni consigliate
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reattività
Human
Immunogeno
Peptide sequence around phosphorylation site of serine 246 / 259 / 220 (T-A-S(p)-EP) derived from Human HDAC4 / HDAC5 / HDAC9.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1.0mg / ml
MW previsto
140 124 111 kd
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Sinonimi
AHO3, BDMR, EC 3.5.1.98, HA6116, HD 4, HD4, HDAC 4, HDAC A, HDAC4_HUMAN, HDACA, Histone deacetylase 4, Histone Deacetylase A, KIAA0288
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9 (Phospho-Ser246/259/220) Antibody #11517 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-HDAC4 (phospho Ser632) Antibody [ARC56258] (A305405) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305405_1.jpg?profile=product_alternative)
![Western Blot - Anti-HDAC4 Antibody [ARC0714] (A307862) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307862_1.jpg?profile=product_alternative)
