Anti-HCN1 Anticorpo [S70] (A304768)

Name: Anti-HCN1 Antibody [S70]
Brand: Antibodies.com
SKU: A304768
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [S70] di topo contro HCN1 per WB, IHC, IP e Antibody Microarray.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-HCN1 Antibody [S70]

Descrizione

Mouse monoclonal [S70] antibody to HCN1.

Applicazioni

WB, IHC, IP, Antibody Microarray

Diluizioni consigliate

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat

Reattività incrociata

This antibody does not cross-react with HCN2.

Immunogeno

Fusion protein amino acids 778-910 (C terminus) of rat HCN1.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

S70

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~100 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

BCNG-1, BCNG1, Brain cyclic nucleotide gated channel 1, Brain cyclic nucleotide-gated channel 1, HAC 2, HAC2, HCN1_HUMAN, Hyperpolarization activated cyclic nucleotide gated potassium channel 1, Potassium channel, voltage-gated, brain, 1, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Immunofluorescence - Anti-HCN1 Antibody [S70] (A304768)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-HCN1 Antibody [S70] (A304768), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN1 Antibody (D) Composite.

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Figure 2: Immunohistochemistry - Anti-HCN1 Antibody [S70] (A304768)

Immunohistochemistry analysis of mouse frozen brain section, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-HCN1 Antibody [S70] (A304768) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature.

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Figure 3: Immunohistochemistry - Anti-HCN1 Antibody [S70] (A304768)

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Figure 4: Immunohistochemistry - Anti-HCN1 Antibody [S70] (A304768)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HCN1 Antibody [S70] (A304768) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.