Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
FLJ39780, FP2025, G protein coupled receptor kinase 5, G protein coupled receptor kinase GRK5, G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5, GPRK5, GRK-pan, GRK5_HUMAN, Pan-GRK
Figure 1: Western Blot - Anti-GRK5 Antibody (A11696)
Western blot analysis of extracts of various cell lines, using Anti-GRK5 Antibody (A11696) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-GRK5 Antibody (A11696) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-GRK5 Antibody (A11696) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-GRK5 Antibody (A11696) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using Anti-GRK5 Antibody (A11696) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
