This antibody recognises the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with ß and ? subunits. Two isoforms of the a subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting.
Applications
WB, ELISA
Dilutions
ELISA: 1:500 - 1:1,000
Reactivity
Mouse, Drosophila
Immunogen
Synthetic peptide sequence C-ANNLRGCGLY corresponding to amino acids 345 - 354 of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.
Sequence
C-ANNLRGCGLY
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein G affinity chromatography.
Concentration
5 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with ß and ? subunits. Two isoforms of the a subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting (Guo et al. 2011).
Synonyms
Guanine nucleotide-binding protein G(o) subunit alpha
