This antibody recognises the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with ß and ? subunits. Two isoforms of the a subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting.

Applicazioni

WB, ELISA

Diluizioni consigliate

ELISA: 1:500 - 1:1,000

Reattività

Mouse, Drosophila

Immunogeno

Synthetic peptide sequence C-ANNLRGCGLY corresponding to amino acids 345 - 354 of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.

Sequenza

C-ANNLRGCGLY

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Protein G affinity chromatography.

Concentrazione

5 mg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conservazione

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Note generali

Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with ß and ? subunits. Two isoforms of the a subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting (Guo et al. 2011).

Sinonimi

DKFZp686O0962, G alpha o, G protein alpha 0, G0 Protein alpha, Galphao, GNAO, GNAO 1, GNAO_HUMAN, Go alpha, Guanine nucleotide binding protein (G protein) alpha activating activity polypeptide O, Guanine nucleotide binding protein alpha activating polypeptide, Guanine nucleotide binding protein G(o) subunit alpha, Guanine nucleotide-binding protein G(o) subunit alpha

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.