Anti-Glutathione Synthetase Anticorpo [ARC0630] (A305338)

Western blot analysis of extracts of various cell lines, using Anti-Glutathione Synthetase Antibody [ARC0630] (A305338) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

Immunofluorescence analysis of NIH-3T3 cells using Anti-Glutathione Synthetase Antibody [ARC0630] (A305338) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).