Anti-Glutathione Peroxidase 4 Anticorpo (A305439)

Name: Anti-Glutathione Peroxidase 4 Antibody
Brand: Antibodies.com
SKU: A305439
Price: 470.0 USD
Availability: InStock

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$470

Anticorpo policlonale di coniglio contro Glutathione Peroxidase 4 per WB e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-Glutathione Peroxidase 4 Antibody

Descrizione

Rabbit polyclonal antibody to Glutathione Peroxidase 4.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 30-197 of human GPX4 (NP_002076.2).

Sequenza

ASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

20 kDa / 22 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

GPX 4, GPX-4, GPX4, GPX4_HUMAN, GSHPx-4, MCSP, mitochondrial, PHGPx, Phospholipid hydroperoxidase, Phospholipid hydroperoxide glutathione peroxidase, Phospholipid hydroperoxide glutathione peroxidase mitochondrial, snGPx, snPHGPx, Sperm nucleus glutathione peroxidase

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-Glutathione Peroxidase 4 Antibody (A305439)

Western blot analysis of extracts of various cell lines, using Anti-Glutathione Peroxidase 4 Antibody (A305439) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Glutathione Peroxidase 4 Antibody (A305439)

Western blot analysis of extracts of Jurkat cells, using Anti-Glutathione Peroxidase 4 Antibody (A305439) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-Glutathione Peroxidase 4 Antibody (A305439)

Western blot analysis of extracts from wild type (WT) and GPX4 knockdown (KD) U-87MG cells, using Anti-Glutathione Peroxidase 4 Antibody (A305439) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunofluorescence - Anti-Glutathione Peroxidase 4 Antibody (A305439)

Immunofluorescence analysis of mouse testis cells using Anti-Glutathione Peroxidase 4 Antibody (A305439) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).