Anti-GLUT2 Anticorpo (A305171)

Name: Anti-GLUT2 Antibody
Brand: Antibodies.com
SKU: A305171
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo policlonale di coniglio contro GLUT2 per WB e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-GLUT2 Antibody

Descrizione

Rabbit polyclonal antibody to GLUT2.

Specificità

Predicted molecular weight at ~57.5 kDa. Observed molecular weight at 60-70 kDa and 38-45 kDa.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:100

Reattività

Human, Rat

Immunogeno

Synthetic peptide from the C-terminal of human GLUT2.

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Peptide affinity purification.

Concentrazione

1 mg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Glucose Transporter 2, Glucose Transporter GLUT2, Glucose transporter type 2, Glucose transporter type 2 liver, Glucose transporter, liver/islet, GLUT-2, GTR2_HUMAN, GTT2, liver, SLC2A2, Solute carrier family 2 (facilitated glucose transporter) member 2, Solute carrier family 2 facilitated glucose transporter member 2, Solute carrier family 2, facilitated glucose transporter member 2

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-GLUT2 Antibody (A305171)

Western blot analysis of human HeLa and HEK293T cell lysates showing detection of ~57.5kDa GLUT2 protein using Anti-GLUT2 Antibody (A305171) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human HeLa (20 µg). Lane 3: human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~72kDa.

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Figure 2: Western Blot - Anti-GLUT2 Antibody (A305171)

Western blot analysis of rat liver showing detection of ~57.5kDa GLUT2 protein using Anti-GLUT2 Antibody (A305171) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~37kDa.

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Figure 3: Immunofluorescence - Anti-GLUT2 Antibody (A305171)

Immunocytochemistry/Immunofluorescence analysis of human colon cancer cell line (HT-29), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GLUT2 Antibody (A305171), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm, membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GLUT2 Antibody (D) Composite.