Anti-Glucocorticoid Receptor Anticorpo [ARC0062] (A305646)

Western blot analysis of extracts of various cell lines, using Anti-Glucocorticoid Receptor Antibody [ARC0062] (A305646) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunoprecipitation analysis of 300µg extracts of U-251MG cells using 3µg of Anti-Glucocorticoid Receptor Antibody [ARC0062] (A305646). This Western blot was performed on the immunoprecipitate using Anti-Glucocorticoid Receptor Antibody [ARC0062] (A305646) at a dilution of 1:1000.