Anti-Ghrelin Anticorpo (A305137)

Name: Anti-Ghrelin Antibody
Brand: Antibodies.com
SKU: A305137
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo policlonale di coniglio contro Ghrelin per WB, ICC/IF e IHC.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-Ghrelin Antibody

Descrizione

Rabbit polyclonal antibody to Ghrelin.

Applicazioni

WB, ICC/IF, IHC

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reattività

Human, Rat

Immunogeno

Synthetic peptide from the mid-protein of Human Ghrelin.

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Peptide affinity purification.

Concentrazione

1 mg/ml

Peso molecolare

~13 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Appetite regulating hormone, Ghrelin 27, Ghrelin 28, Ghrelin and obestatin prepropeptide, Ghrelin/obestatin prepropeptide, ghrl, GHRL_HUMAN, Growth hormone releasing peptide, Growth hormone secretagogue, Growth hormone-releasing peptide, M46 protein, Motilin related peptide, Motilin-related peptide, MTLRP, Obestatin, Protein M46

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Immunohistochemistry - Anti-Ghrelin Antibody (A305137)

Immunohistochemistry analysis of human stomach, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Ghrelin Antibody (A305137) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

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Figure 2: Immunofluorescence - Anti-Ghrelin Antibody (A305137)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Ghrelin Antibody (A305137), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasmic. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ghrelin Antibody. (D) Composite.

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Figure 3: Western Blot - Anti-Ghrelin Antibody (A305137)

Western blot analysis of rat Pancreas showing detection of ~12.9 kDa Ghrelin protein using Anti-Ghrelin Antibody (A305137) at 1:1,000 for 60 minutes at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~12.9 kDa.

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Figure 4: Immunofluorescence - Anti-Ghrelin Antibody (A305137)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Ghrelin Antibody (A305137), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasmic. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ghrelin Antibody. (D) Composite.