This antibody does not cross-react with GFAP-R416W or other proteins - based on KO validation data.
Immunogeno
Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. >50% identity with other proteins (Vimentin, Desmin and Peripherin)..
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
S206B-9
Isotipo
IgG1
Coniugare
Unconjugated
Purificazione
Protein G purification.
Concentrazione
1 mg/ml
Peso molecolare
~50 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GFAP Antibody [S206B-9] (A304763), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GFAP R416WT Antibody. (D) Composite.
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-GFAP Antibody [S206B-9] (A304763), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GFAP R416WT Antibody (D) Composite.
Prodotti alternativi a Anti-GFAP Anticorpo [S206B-9] (A304763)
![Western Blot - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_1.png?profile=product_top)
![Immunocytochemistry/Immunofluorescence - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_2.png?profile=product_top)
![Immunocytochemistry/Immunofluorescence - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_3.png?profile=product_top)
![Western Blot - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_1.png?profile=product_top_thumb)
![Immunocytochemistry/Immunofluorescence - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_2.png?profile=product_top_thumb)
![Immunocytochemistry/Immunofluorescence - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_3.png?profile=product_top_thumb)
![Western Blot - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_1.png?profile=product_image)
![Immunocytochemistry/Immunofluorescence - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_2.png?profile=product_image)
![Immunocytochemistry/Immunofluorescence - Anti-GFAP Antibody [S206B-9] (A304763) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304763_3.png?profile=product_image)
![Immunofluorescence - Anti-GFAP Antibody [5C10] (A85422) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85422_1.jpg?profile=product_alternative)
![Western Blot - Anti-GFAP Antibody [GA-5] - BSA and Azide free (A251887) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251887_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GFAP Antibody [GA-5 + ASTRO/789] (A248708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248709_1.jpg?profile=product_alternative)
![Western Blot - Anti-GFAP Antibody [GA-5] (A248705) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248705_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GFAP Antibody [SPM248] - BSA and Azide free (A251888) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251888_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GFAP Antibody [GA-5 + ASTRO/789] - BSA and Azide free (A251890) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251891_1.jpg?profile=product_alternative)
![Western Blot - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307282_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GFAP Antibody [SPM248] (A248706) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248706_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GFAP Antibody [rASTRO/789] - BSA and Azide free (A251893) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251893_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GFAP Antibody [SPM507] (A248706) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248707_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GFAP Antibody [SPM507] - BSA and Azide free (A251888) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251889_1.jpg?profile=product_alternative)