This antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Applicazioni
WB, IHC-P
Diluizioni consigliate
WB: 1-2 µg/ml, IHC-P: 0.25-0.5 µg/ml
Reattività
Human, Mouse, Rat, Bovine, Porcine, Rabbit, Chicken
Immunogeno
Recombinant GFAP protein.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
ASTRO/789
Isotipo
IgG1
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
200 µg/ml
Peso molecolare
~50 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-GFAP Antibody [ASTRO/789] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.