Anti-GFAP Antibody [5C10] (A85422)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-Galectin 3 Antibody (A104321), at a dilution of 1:2,000, in green, and co-stained with Anti-GFAP Antibody [5C10] (A85422), at a dilution of 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. Certain glial cells express Galectin 3 protein and so appear green, while the majority of glial cells and astrocytes produce GFAP protein and so appear red, and a few cells that express both proteins appear orange-yellow.

Immunohistochemistry analysis of paraffin embedded formalin fixed human cerebellum stained with Anti-GFAP Antibody [5C10] (A85422). The folds of the molecular layer are at the right and granular and white matter layers are on the left. Counterstained with Hematoxylin (blue).

Immunohistochemistry analysis of formalin fixed paraffin embedded adult horse brain samples removed whole, sliced incompletely cross ways and fixed over a 10-20 day period. The whole brain was put in a large necropsy specimen bucket containing 1-2 gallons of fixative, and after 24 hours, the formalin was poured off and then fresh formalin was added. The samples were fixed and embedded in paraffin in 2007 and sections were kept at room temperature until 2011. The sections were processed for antigen retrieval by boiling in Citrate buffer, pH 6, for 10 min. Primary incubation with Anti-GFAP Antibody [5C10] (A85422) was for 1 hour at 37°C, and secondary antibody incubation and color reaction was performed using the Vector mouse ABC kit.