Anti-GDF15 Anticorpo (A84124)

Name: Anti-GDF15 Antibody
Brand: Antibodies.com
SKU: A84124
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab39999). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro GDF15 per ELISA, WB, IHC e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-GDF15 Antibody

Descrizione

Goat polyclonal antibody to GDF15.

Applicazioni

ELISA, WB, IHC, Flow Cytometry

Diluizioni consigliate

ELISA: 1:128,000, WB: 0.1-0.5µg/ul, IHC: 7µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the C terminal region of human GDF15.

Sequenza

C-QKTDTGVSLQTYDD

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

35 kDa

MW previsto

34.1kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

GDF 15, GDF-15, GDF15_HUMAN, Growth differentiation factor 15, Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC 1, Mic-1, MIC1, NAG 1, NAG-1, NAG1, NRG 1, NRG-1, NRG1, NSAID, NSAID (nonsteroidal anti inflammatory drug) activated protein 1, NSAID regulated protein 1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, PLAB, Placental bone morphogenetic protein, Placental bone morphogenic protein, Placental TGF beta, Placental TGF-beta, Prostate differentiation factor, PTGF beta, PTGFB

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Western Blot - Anti-GDF15 Antibody (A84124)

GDF15 expression in Human Prostate lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GDF15 Antibody (A84124) at 0.5µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-GDF15 Antibody (A84124)

GDF15 expression in A549 (A), CaCo-2 (B), and LNCaP (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-GDF15 Antibody (A84124) at 0.1µg/ml (A-B) or 0.3µg/ml (C) and detected by chemiluminescence.

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Figure 3: Western Blot - Anti-GDF15 Antibody (A84124)

GDF15 expression in A549 lysate 2 (A) + peptide (B), CaCo-2 lysate 2 (C) + peptide (D), LNCaP lysate 2 (E) + peptide (F) analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GDF15 Antibody (A84124) at 0.3µg/ml and detected by chemiluminescence.

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Figure 4: Flow Cytometry - Anti-GDF15 Antibody (A84124)

GDF15 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-GDF15 Antibody (A84124) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.