Anti-GATA4 Anticorpo [PCRP-GATA4-1A7] (A314995)

Anti-GATA4 Antibody [PCRP-GATA4-1A7]

Mouse monoclonal [PCRP-GATA4-1A7] antibody to GATA4.

Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4, expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

Flow Cytometry

HeLa or MCF-7 cells.

Flow Cytometry: 1-2 µg/million cells

Human

Recombinant fragment (around Amino Acids 262-321) of human GATA4 protein.

Mouse

Monoclonal

IgG2b

Unconjugated

Protein A/G chromatography.

200 µg/ml

44.57 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

ASD2, GATA 4, GATA binding protein 4, GATA-binding factor 4, GATA4_HUMAN, MGC126629, Transcription factor GATA 4, Transcription factor GATA-4, Transcription factor GATA4, VSD1