The antibody recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).

Applicazioni

WB, ICC

Diluizioni consigliate

WB: 1-2 µg/ml; Reducing conditions, IHC: Methanol/acetone fixation required.

Reattività

Protozoa, Plants, Animals

Immunogeno

Human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449.

Sequenza

EYHAATRPDYISWGTQ

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

TU-32

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein A chromatography.

Concentrazione

1 mg/ml

MW previsto

48 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Gamma 1 tubulin, Gamma 2 tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma tubulin complex component 1, Gamma-2-tubulin, GCP 1, GCP1, MGC131994, TBG2_HUMAN, TUBG, TUBG1, TUBG2, TUBGCP1, Tubulin gamma 1 chain, Tubulin gamma 2 chain, Tubulin gamma complex-associated protein 1, Tubulin gamma-2 chain, tubulin, gamma 1, tubulin, gamma 2, tubulin, gamma polypeptide, Xgam

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (1)

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Figure 1: Western Blot - Anti-gamma Tubulin Antibody [TU-32] (A86251)

Differential reactivity of monoclonal antibodies to ?-tubulin with human ?-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human ?-tubulin 1 (?-Tb1) or ?-tubulin 2 (?-Tb2), probed with Abs to ?-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of ?-Tb1 or ?-Tb2 probed with Abs to ?-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.