Anti-G3BP Antibody [ARC0875] (A305824)

Name: Anti-G3BP Antibody [ARC0875]
Brand: Antibodies.com
SKU: A305824
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0875] antibody to G3BP for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-G3BP Antibody [ARC0875]

Description

Rabbit monoclonal [ARC0875] antibody to G3BP.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human G3BP1 (Q13283).

Sequence

EVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0875

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ATP-dependent DNA helicase VIII, G3BP-1, GAP SH3 domain-binding protein 1, hDH VIII, Ras GTPase-activating protein-binding protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-G3BP Antibody [ARC0875] (A305824)

Western blot analysis of extracts of various cell lines, using Anti-G3BP Antibody [ARC0875] (A305824) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunofluorescence - Anti-G3BP Antibody [ARC0875] (A305824)

Immunofluorescence analysis of HeLa cells using Anti-G3BP Antibody [ARC0875] (A305824) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-G3BP Antibody [ARC0875] (A305824)

Immunofluorescence analysis of NIH-3T3 cells using Anti-G3BP Antibody [ARC0875] (A305824) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-G3BP Antibody [ARC0875] (A305824)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-G3BP Antibody [ARC0875] (A305824). This Western blot was performed on the immunoprecipitate using Anti-G3BP Antibody [ARC0875] (A305824) at a dilution of 1:1000.

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