Anti-FGF13 Anticorpo [S235-22] (A304756)

Name: Anti-FGF13 Antibody [S235-22]
Brand: Antibodies.com
SKU: A304756
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [S235-22] di topo contro FGF13 per WB, IHC e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-FGF13 Antibody [S235-22]

Descrizione

Mouse monoclonal [S235-22] antibody to FGF13.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:1,000

Reattività

Human, Mouse, Rat

Reattività incrociata

This antibody does not cross-react with FGF13B/FHF2B, but does cross-react with FGF12A/FHF1A and FGF14A/FHF4A.

Immunogeno

Synthetic peptide amino acids 2-18 (AAAIASSLIRQKRQARE) of human FHF2A. 100% identical to rat, 94% identical to mouse. >80% identity with FGF12A/FHF1A, FGF14A/FHF4A and FGF11A/FHF3A

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

S235-22

Isotipo

IgG2b

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~30 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

FGF 13, FGF 2, FGF-13, FGF13_HUMAN, FGF2, FHF 2, FHF-2, FHF2, Fibroblast growth factor 13, Fibroblast growth factor homologous factor 2, OTTHUMP00000024143, OTTHUMP00000024144

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-FGF13 Antibody [S235-22] (A304756)

Western blot analysis of rat brain membrane showing detection of ~30 kDa FGFA/FHFA (pan) protein using Anti-FGF13 Antibody [S235-22] (A304756) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~30 kDa.

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Figure 2: Immunofluorescence - Anti-FGF13 Antibody [S235-22] (A304756)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-FGF13 Antibody [S235-22] (A304756), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cell Projection, Nucleus, Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) FGFA/FHFA (pan) Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-FGF13 Antibody [S235-22] (A304756)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-FGF13 Antibody [S235-22] (A304756), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) FGFA/FHFA (pan) Antibody (D) Composite.