Anti-FACL4 Anticorpo (A83246)

Name: Anti-FACL4 Antibody
Brand: Antibodies.com
SKU: A83246
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab110007). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro FACL4 per ELISA, WB, IHC, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-FACL4 Antibody

Descrizione

Goat polyclonal antibody to FACL4.

Applicazioni

ELISA, WB, IHC, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:128,000, WB: 0.1-0.3µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the C terminal region of human FACL4.

Sequenza

C-HYLKDIERMYGGK

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

80-85 kDa

MW previsto

79.2kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

ACS 4, ACS4, ACSL 4, Acsl4, ACSL4_HUMAN, acyl CoA synthetase 4, Acyl CoA synthetase long chain family member 4, FACL 4, Fatty acid Coenzyme A ligase, fatty acid Coenzyme A ligase long-chain 4, LACS 4, LACS4, Lignoceroyl CoA synthase, Long chain 4, long chain acyl CoA synthetase 4, long chain fatty acid CoA ligase 4, long chain fatty acid Coenzyme A ligase 4, Long-chain acyl-CoA synthetase 4, Long-chain-fatty-acid--CoA ligase 4, MRX63, MRX68

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-FACL4 Antibody (A83246)

FACL4 expression in HepG2 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-FACL4 Antibody (A83246) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-FACL4 Antibody (A83246)

FACL4 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-FACL4 Antibody (A83246) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-FACL4 Antibody (A83246)

FACL4 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-FACL4 Antibody (A83246) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunohistochemistry - Anti-FACL4 Antibody (A83246)

FACL4 expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-FACL4 Antibody (A83246) at 3.75µg/ml and revealed with alkaline phosphatase (AP).