Western blot analysis of extracts of various cell lines, using Anti-FABP6 Antibody (A12036) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of mouse small intestine cells using Anti-FABP6 Antibody (A12036) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
Immunofluorescence analysis of rat small intestine cells using Anti-FABP6 Antibody (A12036) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
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