Rabbit polyclonal antibody to Ezrin (Phospho-Tyr478)
Specificity
The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 478.
Applications
WB
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 478 (P-V-Y(p)-E-P) derived from Human Ezrin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from K562 cells treated with Na3VO4 using Phospho-Ezrin (Tyr478) antibody #11908.The lane on the right is treated with the antigen-specific peptide.
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