Anti-Estrogen Receptor beta Anticorpo (A83108)

Name: Anti-Estrogen Receptor beta Antibody
Brand: Antibodies.com
SKU: A83108
Price: 480.0 USD
Availability: InStock

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$480

Anticorpo policlonale di capra contro Estrogen Receptor beta per ELISA, WB e IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-Estrogen Receptor beta Antibody

Descrizione

Goat polyclonal antibody to Estrogen Receptor beta.

Applicazioni

ELISA, WB, IF

Diluizioni consigliate

ELISA: 1:128,000, WB: 1-3µg/ml, IF: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region, amino acids 417-428, of human Estrogen Receptor beta.

Sequenza

C-TQDADSSRKLAH

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

50 kDa

MW previsto

53.0kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

ER BETA, ER-beta, Erb, ESR B, ESR BETA, ESR2, ESR2_HUMAN, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, Estrogen receptor 2, estrogen receptor 2 (ER beta), estrogen receptor beta 4, NR3A2, Nuclear receptor subfamily 3 group A member 2

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in Human Placenta lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Weak cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.