Anti-Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) Antibody [G15-B] (A8261)

Name: Anti-Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) Antibody [G15-B]
Brand: Antibodies.com
SKU: A8261
Price: 475.0 USD
Availability: InStock

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$475

Rabbit monoclonal (G15-B) antibody to Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) for WB, ELISA, IP and ICC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) Antibody [G15-B]

Description

Rabbit monoclonal (G15-B) antibody to Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187).

Applications

WB, ELISA, IP, ICC

Dilutions

WB: 1:5,000, ELISA: 1:20,000-1:100,000.

Reactivity

Human, Mouse, Rat

Immunogen

Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk1 + Erk2.

Host

Rabbit

Clonality

Monoclonal

Clone ID

G15-B

Isotype

IgG

Conjugate

Unconjugated

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 8.0, with 10 mg/ml BSA and 0.05% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK-2, ERT1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, p42-MAPK, PRKM1, PRKM2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) Antibody [G15-B] (A8261)

Western blot analysis of Erk 1,2 activation in untreated PC12 cells (A), cells treated with EGF (100ng/ml | 5min) (B), PMA (100nM | 30min) (C) in serum free DMEM, and EGF (100ng/ml | 5min) with 10% FBS in DMEM (D). Wells were equally loaded with 50µg of whole cell lysate proteins/well.

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Immunocytochemistry - Anti-Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) Antibody [G15-B] (A8261)

Representative pictures of phospho-Erk1,2 expression in HEK293 cells, (untreated cells) visualized with Anti-Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) Antibody (A8261). Primary antibody dilution: 1:100.

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Immunocytochemistry - Anti-Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) Antibody [G15-B] (A8261)

Representative pictures of phospho-Erk1,2 expression in HEK293 cells, (hydrogen peroxide treated cells) visualized with Anti-Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) Antibody (A8261). Primary antibody dilution: 1:100.

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