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Anti-ERK1 + ERK2 Anticorpo (A87643)

Nome
Anti-ERK1 + ERK2 Antibody
Descrizione
Rabbit polyclonal antibody to ERK1 + ERK2.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK1 / ERK2 (NP_620407.1/NP_002737.2).
Sequenza
LNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
42 kDa / 44 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
ERK 1, ERK 2, ERK-2, ERK1, erk1/2, ERK2, ERT1, ERT2, Extracellular signal regulated kinase 1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Mapk1, MAPK2, MAPK3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01_HUMAN, p38, p40, p41, p42-MAPK, PRKM 2
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-ERK1 + ERK2 Antibody (A87643) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-ERK1 + ERK2 Antibody (A87643)
Western blot analysis of extracts of various cell lines, using Anti-ERK1 + ERK2 Antibody (A87643) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-ERK1 + ERK2 Antibody (A87643) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-ERK1 + ERK2 Antibody (A87643)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-ERK1 + ERK2 Antibody (A87643) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-ERK1 + ERK2 Antibody (A87643) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-ERK1 + ERK2 Antibody (A87643)
Immunohistochemistry analysis of paraffin-embedded human uterine cancer using Anti-ERK1 + ERK2 Antibody (A87643) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-ERK1 + ERK2 Antibody (A87643) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-ERK1 + ERK2 Antibody (A87643)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ERK1 + ERK2 Antibody (A87643) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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