Anti-ERK1 Anticorpo (A83709)

Name: Anti-ERK1 Antibody
Brand: Antibodies.com
SKU: A83709
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Novus (NB100-1508). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro ERK1 per ELISA, WB, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-ERK1 Antibody

Descrizione

Goat polyclonal antibody to ERK1.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:4,000, WB: 0.1-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Reattività incrociata

This antibody is not expected to cross react with ERK2

Immunogeno

Synthetic peptide corresponding to the N terminal region of human ERK1.

Sequenza

GGEPRRTEGVGP-C

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

40 kDa

MW previsto

40.1kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

ERK, ERK 1, ERK-1, ERT 2, ERT2, Extracellular Signal Regulated Kinase 1, Extracellular signal related kinase, Extracellular signal related kinase 1, Extracellular signal-regulated kinase 1, HGNC6877, HS44KDAP, HUMKER1A, Insulin Stimulated MAP2 Kinase, Insulin-stimulated MAP2 kinase, MAP Kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK, MAPK 1, MAPK 3, MAPK1, Mapk3, MGC20180, Microtubule Associated Protein 2 Kinase, Microtubule-associated protein 2 kinase, Mitogen Activated Protein Kinase 3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, MK03_HUMAN, OTTHUMP00000174538, OTTHUMP00000174541, p44 ERK1, p44 MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM 3, PRKM3, Protein Kinase Mitogen Activated 3

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-ERK1 Antibody (A83709)

ERK1 expression in Human Cerebellum (A) and Frontal Cortex (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ERK1 Antibody (A83709) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-ERK1 Antibody (A83709)

ERK1 expression in A431 (A) and HepG2 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody was performed with Anti-ERK1 Antibody (A83709) at 1µg/ml (A) or 0.3µg/ml (B) and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-ERK1 Antibody (A83709)

ERK1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ERK1 Antibody (A83709) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-ERK1 Antibody (A83709)

ERK1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ERK1 Antibody (A83709) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.