Anti-EPO-R Anticorpo (A283241)

Anti-EPO-R Antibody

Sheep polyclonal antibody to EPO-R.

This antibody recognises the human Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival. Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function. Sheep anti Human erythropoietin receptor antibody (AHP1825) blocks Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cells. Sheep anti Human erythropoietin receptor antibody is suitable for use in immunocytochemistry and for use in functional assays. Removal of sodium azide is recommended prior to use in functional assays.

WB, ELISA, IP

ELISA: 50ng/ml, IP: 10 µg/ml, WB: 10 µg/ml

Human, Mouse

Partial cDNA of the extracellular domain of human EPOR expressed as a GST fusion protein in E. Coli.

Sheep

Polyclonal

IgG

1 mg/ml

49 kDa for EPOR-GST on SDS-PAGE.

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

Sheep anti Human erythropoietin receptor antibody recognizes the human Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival. Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function. Sheep anti Human erythropoietin receptor antibody (AHP1825) blocks Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cells (Harris et al. 1992).Sheep anti Human erythropoietin receptor antibody is suitable for use in immunocytochemistry and for use in functional assays. Removal of sodium azide is recommended prior to use in functional assays.

EPO R, EPO Receptor, epor, EPOR_HUMAN, Erythropoietin receptor, Erythropoietin receptor precursor, MGC138358