The antibody detects endogenous levels of EPHA2 / 3 / 4 only when phosphorylated at tyrosine 588 / 596
Applicazioni
WB, IF
Diluizioni consigliate
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reattività
Human, Mouse
Immunogeno
Peptide sequence around phosphorylation site of tyrosine 588 / 596 (K-T-Y(p)-V-R) / (R-T-Y(p)-V-D) derived from Human EPHA2 / 3 / 4.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1.0mg / ml
Formulazione
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody #11690.The lane on the right is treated with the antigen-specific peptide.
![SDS-PAGE - Anti-Eph Receptor A2 Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A324011) - Antibodies.com](https://cdn.antibodies.com/image/catalog/324/A324011_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Eph Receptor A2 Antibody [MEDI-547] - Low endotoxin, Azide free (A324012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/324/A324012_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-Eph Receptor A2 Antibody [DM167] - BSA and Azide free (A318542) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318542_1.jpg?profile=product_alternative)
