Anti-EpCAM Anticorpo [EGP40/1372] (HRP) (A277522)

Anti-EpCAM Antibody [EGP40/1372] (HRP)

Mouse monoclonal [EGP40/1372] antibody to EpCAM (HRP).

This antibody is directly conjugated to Horse Radish Peroxidase (HRP) enzyme. Compared to indirect-primary-secondary detection system, it offers the following advantages: 1) Assay time is shorter as only one antibody is involved, 2) Background is reduced as there is no secondary antibody, and 3) It can be used to detect the target protein in the antibody-host samples (i.e. Mouse-on-Mouse or Rabbit-on-Rabbit), avoiding problem related to host-immunoglobulins.Epitope of this MAb is mapped between aa 202-209 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

Recombinant fragment from the extracellular domain, around amino acids 100-224, of human EpCAM protein. The exact sequence is proprietary.

Mouse

Monoclonal

EGP40/1372

HRP

Protein A/G chromatography.

100 µg/ml

40-43 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% ProClin 300.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

17 1A, 323/A3, Adenocarcinoma associated antigen, Adenocarcinoma-associated antigen, Antigen identified by monoclonal, AUA1, CD326, CD326 antigen, Cell surface glycoprotein Trop 1, Cell surface glycoprotein Trop 2, Cell surface glycoprotein Trop-1, CO 17A, CO17 1A, CO17A, DIAR5, EGP, EGP 2, EGP2, EGP314, EGP40, Ep CAM, Ep-CAM, EpCAM1, EPCAM_HUMAN, Epithelial cell adhesion molecule, Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD), Epithelial cell surface antigen, Epithelial cellular adhesion molecule, Epithelial glycoprotein, Epithelial glycoprotein 1, Epithelial glycoprotein 314, ESA, GA733 1, GA733 2, GA733-2, gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein, gp4, hEGP 2, hEGP314, HNPCC8, Human epithelial glycoprotein 2, KS 1/4 antigen, KS1/4, KSA, Ly74, Lymphocyte antigen 74, M1S 1, M1S2, M4S1, Major gastrointestinal tumor associated protein GA733 2, Major gastrointestinal tumor-associated protein GA733-2, mEGP314, Membrane component chromosome 4 surface marker (35kD glycoprotein), Membrane component, chromosome 4, surface marker, Membrane component, chromosome 4, surface marker 1, MIC18, MK 1, Protein 289A, TACD1, TACSTD1, TROP1, Tumor associated calcium signal transducer 1, Tumor associated calcium signal transducer 2 precursor, Tumor-associated calcium signal transducer 1

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