Anti-Eotaxin Antibody [1.3_17H09-02C06] - BSA and Azide free (A278924)

Anti-Eotaxin Antibody [1.3_17H09-02C06] - BSA and Azide free

Mouse monoclonal [1.3_17H09-02C06] antibody to Eotaxin.

This antibody recognises C-C motif chemokine 11 (CCL11), also known as eotaxin, eosinophil chemotactic protein and small inducible cytokine A11. CCL11 is produced by a range of cell types including epithelial cells, fibroblasts, smooth muscle cells, astrocytes, chondrocytes and tissue resident macrophages. Additionally, in the central nervous system, it is expressed by choroid plexus epithelial cells, pericytes, and microglia under inflammatory conditions. CCL11 acts as a chemoattractant for eosinophil recruitment to inflammatory sites, including during asthma attacks. Upon binding of CCL11 to its receptor CCR3, a series of signaling events are triggered which can result in calcium mobilization, CD11b upregulation, MAPK activation and actin polymerization. It can also result in a rapid change of shape associated with chemotaxis and granule release.

WB, ELISA, Flow Cytometry

ELISA: 2.0 µg/ml - 1 µg/ml, WB: 0.25 µg/ml - 0.5 µg/ml

Human

E. coli derived recombinant human CCL11.

Mouse

Monoclonal

1.3_17H09-02C06

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Lyophilized

Reconstitute with 0.5 ml of distilled water.

Supplied in Phosphate Buffered Saline.

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human CCL11 antibody, clone 1.3_17H09-02C06, recognizes C-C motif chemokine 11 (CCL11), also known as eotaxin, eosinophil chemotactic protein and small inducible cytokine A11. CCL11 is produced by a range of cell types including epithelial cells, fibroblasts, smooth muscle cells, astrocytes, chondrocytes and tissue resident macrophages. Additionally, in the central nervous system, it is expressed by choroid plexus epithelial cells, pericytes, and microglia under inflammatory conditions (Teixeira et al. 2018). CCL11 acts as a chemoattractant for eosinophil recruitment to inflammatory sites, including during asthma attacks (Wen & Rothenberg 2016). Upon binding of CCL11 to its receptor CCR3, a series of signaling events are triggered which can result in calcium mobilization, CD11b upregulation, MAPK activation and actin polymerization. It can also result in a rapid change of shape associated with chemotaxis and granule release (Conroy & Williams 2001).

C-C motif chemokine 11, CCL11, Eosinophil chemotactic protein, SCYA11, Small-inducible cytokine A11