Anti-eNOS (phospho Ser1177) Anticorpo (A10995)

Name: Anti-eNOS (phospho Ser1177) Antibody
Brand: Antibodies.com
SKU: A10995
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro eNOS (phospho Ser1177) per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-eNOS (phospho Ser1177) Antibody

Descrizione

Rabbit polyclonal antibody to eNOS (phospho Ser1177).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reattività

Human, Mouse

Immunogeno

A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).

Sequenza

TQSFS

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

133 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

cNOS, Constitutive NOS, EC NOS, EC-NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, Nitric oxide synthase 3, Nitric oxide synthase 3 (endothelial cell), Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, Nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III, NOS3, NOS3_HUMAN, NOSIII

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-eNOS (phospho Ser1177) Antibody (A10995)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-eNOS (phospho Ser1177) Antibody (A10995) at 1:1,000 dilution. NIH/3T3 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-eNOS (phospho Ser1177) Antibody (A10995)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-eNOS (phospho Ser1177) Antibody (A10995) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-eNOS (phospho Ser1177) Antibody (A10995)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-eNOS (phospho Ser1177) Antibody (A10995) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.