Figure 1: Western Blot - Anti-eIF4G1 Antibody (A10162)
Western blot analysis of extracts of HeLa cells, using Anti-eIF4G1 Antibody (A10162) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-eIF4G1 Antibody (A10162) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 3: Western Blot - Anti-eIF4G1 Antibody (A10162)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-eIF4G1 Antibody (A10162). This Western blot was performed on the immunoprecipitate using Anti-eIF4G1 Antibody (A10162) at a dilution of 1:500.
Prodotti alternativi a Anti-eIF4G1 Anticorpo (A10162)
