Rabbit polyclonal antibody to eIF4G (phospho-Ser1231)
Specificità
The antibody detects endogenous level of eIF4G only when phosphorylated at serine 1231.
Applicazioni
WB, IHC
Diluizioni consigliate
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reattività
Human
Immunogeno
Peptide sequence around phosphorylation site of serine 1231 (P-V-S(p)-P-L) derived from Human eIF4G.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1.0mg / ml
MW previsto
220kd
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (phospho-Ser1231) Antibody #11514 (left) or the same antibody preincubated with blocking peptide (right).
Prodotti alternativi a Anti-eIF4G (phospho-Ser1231) Anticorpo (A50399)
