Anti-eIF4EBP1 (phospho Thr70) Anticorpo [ARC53785] (A308921)

Name: Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785]
Brand: Antibodies.com
SKU: A308921
Price: 540.0 USD
Availability: InStock

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$540

Anticorpo monoclonale [ARC53785] di coniglio contro eIF4EBP1 (phospho Thr70) per WB.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785]

Descrizione

Rabbit monoclonal [ARC53785] antibody to eIF4EBP1 (phospho Thr70).

Applicazioni

Diluizioni consigliate

WB: 1:2,000-1:6,000

Reattività

Human, Mouse

Immunogeno

A synthetic phosphorylated peptide around T70 of human Phospho-eIF4EBP1-T70 (NP_004086.1).

Sequenza

TKTPP

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC53785

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

15 - 20 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (2)

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Figure 1: Western Blot - Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921)

Western blot analysis of extracts of various cell lines, using Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921) at 1:5,000 dilution. 293T cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Western Blot - Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921)

Western blot analysis of extracts of NIH/3T3, using Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921) at 1:5,000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.