Rabbit polyclonal antibody to eIF4EBP1 (phospho Ser65).
Applicazioni
WB, IP
Diluizioni consigliate
WB: 1:500-1:1,000, IP: 1:50-1:100
Reattività
Human
Immunogeno
A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65 (NP_004086.1).
Sequenza
RNSPV
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Affinity purification.
Peso molecolare
20 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Figure 1: Western Blot - Anti-eIF4EBP1 (phospho Ser65) Antibody (A16404)
Western blot analysis of extracts of 293T cells, using Anti-eIF4EBP1 (phospho Ser65) Antibody (A16404) at 1:1,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Prodotti alternativi a Anti-eIF4EBP1 (phospho Ser65) Anticorpo (A16404)
