Synthetic peptide from the mid-protein of Human 4EBP1 (aa. 50-80).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
12.6 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
4E-BP1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~12.6 kDa 4EBP1 protein using Anti-eIF4EBP1 Antibody (A304867) at 1:500 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~12.6 kDa.
Immunohistochemistry analysis of human breast cancer, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-eIF4EBP1 Antibody (A304867) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Localization: Cytoplasm. Magnification: 10X. HRP-DAB Detection.
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