Anti-eIF4E (Phospho-Ser209) Antibody (A50182)

Anti-eIF4E (Phospho-Ser209) Antibody

Rabbit polyclonal antibody to eIF4E (Phospho-Ser209)

The antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.

WB, IHC, IF

Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200

Human

Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.

Rabbit

Polyclonal

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/ml

25kd

Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

eIF-4E, eIF-4F 25 kDa subunit, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein