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Anti-EIF2S1 (phospho Ser51) Anticorpo (A89604)

Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604) - Antibodies.com Immunofluorescence - Anti-EIF2S1 (phospho Ser51) Antibody (A89604) - Antibodies.com Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604) - Antibodies.com
$435
Product Datasheet

Anticorpo policlonale di coniglio contro EIF2S1 (phospho Ser51) per WB, ICC/IF e IP.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
5-8 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-EIF2S1 (phospho Ser51) Antibody
Descrizione
Rabbit polyclonal antibody to EIF2S1 (phospho Ser51).
Applicazioni
WB, ICC/IF, IP
Diluizioni consigliate
WB: 1:1,000-1:2,000, IP: 1:50-1:100, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic phosphorylated peptide around S51 of human eIF2a (NP_004085.1).
Sequenza
SELSR
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
38 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
EIF 2, EIF 2 alpha, EIF 2A, EIF 2alpha, eIF-2-alpha, eIF-2A, EIF-2alpha, EIF2, EIF2 alpha, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, Eukaryotic translation initiation factor 2 subunit alpha, IF2A_HUMAN
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)
Western blot analysis of extracts of HeLa cells, using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at 1:2,000 dilution or EIF2S1 antibody (A0764). HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-EIF2S1 (phospho Ser51) Antibody (A89604) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunofluorescence - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)
Immunofluorescence analysis of HeLa and HeLa treated with CA cells using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-EIF2S1 (phospho Ser51) Antibody (A89604). This Western blot was performed on the immunoprecipitate using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at a dilution of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.

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