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Anti-EIF2B2 Anticorpo (A15496)

Nome
Anti-EIF2B2 Antibody
Descrizione
Rabbit polyclonal antibody to EIF2B2.
Applicazioni
WB, IHC, ICC/IF, IP
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human EIF2B2 (NP_055054.1).
Sequenza
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNE
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
39 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
EI2BB_HUMAN, EIF 2Bbeta, EIF-2Bbeta, EIF2B, EIF2B GDP GTP exchange factor subunit beta, EIF2BB, Eukaryotic translation initiation factor 2B, Eukaryotic translation initiation factor 2B beta, Eukaryotic translation initiation factor 2B, subunit 2, Eukaryotic translation initiation factor 2B, subunit 2 beta, Eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa, S20I15, S20III15, Translation initiation factor eIF-2B subunit beta
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-EIF2B2 Antibody (A15496) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-EIF2B2 Antibody (A15496)
Western blot analysis of extracts of various cell lines, using Anti-EIF2B2 Antibody (A15496) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-EIF2B2 Antibody (A15496) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-EIF2B2 Antibody (A15496)
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-EIF2B2 Antibody (A15496) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-EIF2B2 Antibody (A15496) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-EIF2B2 Antibody (A15496)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-EIF2B2 Antibody (A15496) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-EIF2B2 Antibody (A15496) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-EIF2B2 Antibody (A15496)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-EIF2B2 Antibody (A15496) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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