Figure 1: Western Blot - Anti-E2F6 Antibody (A15091)
Western blot analysis of extracts of various cell lines, using Anti-E2F6 Antibody (A6151) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg Anti-E2F6 Antibody (A6151). Western blot was performed from the immunoprecipitate using Anti-E2F6 Antibody (A6151) at a dilition of 1:500.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Anti-E2F6 Antibody (A6151) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.