Anti-Doublecortin Anticorpo (A83146)

Name: Anti-Doublecortin Antibody
Brand: Antibodies.com
SKU: A83146
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab113435). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro Doublecortin per ELISA, WB, IHC e IF.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Tag dei prodotti:

Neuronal Marker

Descrizione Prodotto

Nome

Anti-Doublecortin Antibody

Descrizione

Goat polyclonal antibody to Doublecortin.

Applicazioni

ELISA, WB, IHC, IF

Diluizioni consigliate

ELISA: 1:32,000, WB: 0.01-0.03µg/ml, IHC: 2-4µg/ml, IF: 5-10µg/ml

Reattività

Human, Mouse, Rat

Immunogeno

Synthetic peptide corresponding to the internal region, amino acids 232-242, of human Doublecortin.

Sequenza

C-KTSANMKAPQS

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

45 kDa

MW previsto

49.3kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

DBCN, Dbct, DC, DCX, DCX_HUMAN, Doublecortex, Doublin, FLJ51296, Lis X, Lis-X, Lissencephalin X, Lissencephalin-X, Lissencephaly X linked, Lissencephaly X linked doublecortin, LISX, Neuronal migration protein doublecortin, OTTHUMP00000023859, OTTHUMP00000023860, OTTHUMP00000216315, OTTHUMP00000216316, SCLH, XLIS

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Immunofluorescence - Anti-Doublecortin Antibody (A83146)

Doublecortin expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Doublecortin Antibody (A83146) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-Doublecortin Antibody (A83146)

Doublecortin expression in KNRK cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Doublecortin Antibody (A83146) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunohistochemistry - Anti-Doublecortin Antibody (A83146)

Doublecortin expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-Doublecortin Antibody (A83146) at 5µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 4: Immunohistochemistry - Anti-Doublecortin Antibody (A83146)

Negative control for Doublecortin expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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