Western Blot - Anti-DOK2 (phospho Tyr299) Antibody (A10905)
Western blot analysis of extracts from Jurkat cells using Anti-DOK2 (phospho Tyr299) Antibody (A10905). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-DOK2 (phospho Tyr299) Antibody (A10905). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.